TB-500 Peptide (Thymosin Beta-4) – High Purity for Research – Wholesale
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High-purity TB-500 peptide (Ac-LKKTETQ), a synthetic analog of thymosin beta-4’s active actin-binding domain. Lyophilized powder. ≥98% purity. CAS 885340-08-9. For research use only. Bulk wholesale available with global shipping.Thymosin beta-4 peptide
TB-500 Peptide (Thymosin Beta-4) – High Purity for Research – Wholesale
TB-500 is a synthetic peptide corresponding to the active actin-binding region of the naturally occurring protein thymosin β₄ (Tβ₄). The peptide segment Ac-LKKTETQ (residues 17–23 of the full 43-amino-acid Tβ₄ sequence) is the conserved domain responsible for actin binding, cell migration, and tissue repair mechanisms . By acetylating the N-terminus, the synthetic TB-500 analog exhibits enhanced stability and bioavailability compared to the native fragment .
Scientific Background
Thymosin β₄ is a 43-amino-acid polypeptide (molecular weight: 4,921 Da) found in nearly all mammalian cells, with the highest concentrations in platelets, leukocytes, and wound fluid . The protein plays a critical role in cytoskeletal dynamics, cell motility, and tissue repair through its ability to sequester G-actin monomers and regulate the equilibrium between globular and filamentous actin .
The synthetic peptide TB-500 (fequesetide) preserves this actin-binding functionality in a truncated 7-amino-acid format (molecular weight: 889 Da), offering researchers a tool to study actin-mediated cellular processes without the complexity of the full-length protein .
Key Features
| Feature | Detail |
|---|---|
| 🧬 Sequence | Ac-LKKTETQ (N-terminally acetylated) |
| 🔬 Molecular Formula | C₃₈H₆₈N₁₀O₁₄ |
| ⚖️ Molecular Weight | 889.01 Da |
| 🧪 Purity | ≥98% (HPLC) – up to 99.9% available from premium sources |
| 🔢 CAS Number | 885340-08-9 |
| ❄️ Storage (Powder) | -20°C (stable for up to 3 years) |
| ❄️ Storage (Solution) | -20°C (stable for up to 6 months); avoid freeze-thaw cycles |
| Available Formats | Lyophilized powder in vials (2mg, 5mg, 10mg, bulk) |
| Research Applications | Actin polymerization studies, cell migration assays, wound healing models, angiogenesis research |
Mechanism of Action (Research Context)
TB-500 functions primarily through its interaction with G-actin (globular actin). The LKKTET motif binds to G-actin in a 1:1 stoichiometry, sequestering actin monomers and preventing their polymerization into F-actin (filamentous actin) . This equilibrium is described by:
F-actin ⇌ G-actin + TB-500 ⇌ G-actin/TB-500 complex
By maintaining a reservoir of polymerizable actin monomers, TB-500 regulates:
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Cytoskeletal dynamics – Cell shape and structural integrity
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Cell migration – Keratinocyte and endothelial cell motility
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Tissue repair mechanisms – In vitro wound healing models
Additionally, TB-500 has been shown to upregulate matrix metalloproteinases (MMP-2, MMP-9), facilitating extracellular matrix remodeling during cell migration studies .
Research Applications
| Application Area | Description |
|---|---|
| 🧫 Cell Migration Assays | Studying keratinocyte and endothelial cell motility |
| 🔬 Actin Polymerization Studies | Investigating cytoskeletal dynamics and G-actin sequestration |
| 🩹 Wound Healing Models | In vitro and in vivo tissue repair research |
| Angiogenesis Research | Endothelial cell differentiation and blood vessel formation |
| 🔧 Tissue Regeneration | Collagen deposition and fibroblast activity studies |
| 🧪 Inflammation Research | Investigating anti-inflammatory pathways |
In vitro studies have demonstrated that TB-500 (15–25 μg/ml) promotes cell migration and wound healing by enhancing ATP-induced intracellular calcium ion increase and activating purinergic P2 receptors . In vivo research using BALB/c mouse skin wound models showed that topical administration of TB-500 (15 μg/30 μl, once daily for 10 days) significantly accelerates wound healing .
Specifications – Bulk/Wholesale Format
| Specification | Detail |
|---|---|
| Product Form | Lyophilized (freeze-dried) white powder |
| Purity | ≥98% (HPLC) – ≥99.8% available from GMP-certified suppliers |
| CAS Number | 885340-08-9 |
| Molecular Weight | 889.01 Da |
| Storage Temperature | -20°C (long-term); room temperature acceptable for short-term (≤30 days) |
| Shelf Life (Unopened) | 24 months at room temperature; 36 months at 2-8°C |
| Shelf Life (Reconstituted) | 7 days at 2-8°C (do not freeze/thaw repeatedly) |
| Country of Origin | Available from GMP-certified facilities (China, USA, Europe) |
Handling & Reconstitution Instructions (For Research Use Only)
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Reconstitution: Add bacteriostatic water or sterile saline to the lyophilized powder. A standard ratio is 1 mL water per 1 mg peptide .
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Mixing: Gently swirl or stir. Do not shake vigorously, as peptides are sensitive to mechanical stress .
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Storage After Reconstitution: Store at 2-8°C and use within 7 days. Avoid repeated freeze-thaw cycles .
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For Difficult Dissolution: Add a small amount of DMSO first, then dilute with water .
Compliance & Disclaimer
⚠️ FOR RESEARCH USE ONLY. NOT FOR HUMAN OR VETERINARY USE.
This product is sold for laboratory research purposes only. It is not a pharmaceutical, drug, or medical device. Not intended for diagnostic, therapeutic, or in vivo human applications. Not for use in food, cosmetics, or household products.
TB-500 is recognized as a prohibited substance in equine and human sports by the International Federation of Horseracing Authorities and the World Anti-Doping Agency (WADA) . Any use in competitive animals or humans is strictly prohibited.
By purchasing this product, you affirm that you are a qualified research professional and that you will use this product only in lawful, ethical research applications.
Why Source From Us?
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✅ High-purity peptides (≥98% to 99.9% by HPLC)
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✅ GMP-certified manufacturing facilities (ISO9001, ISO22000)
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✅ Third-party COA (Certificate of Analysis) available for each batch
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✅ Bulk wholesale pricing – no MOQ for regular items
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✅ OEM/ODM services available (custom purity, size, packaging)
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✅ Discreet worldwide shipping via DHL, FedEx, UPS (10-15 days delivery)
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✅ Free samples available (1-2 vials, buyer pays shipping)
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✅ Refund/replacement policy for quality issues
Contact us for wholesale pricing, COA requests, bulk quotations, and shipping details. Long‑term research supply partnerships welcome.
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