Thymosin Beta-4 (Tβ4) – Full-Length 43-Amino Acid Peptide – High Purity Lyophilized Powder – For Research Use Only
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High-purity full-length Thymosin Beta-4 (Tβ4) peptide, 43 amino acids (Ac-SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES). ≥98% purity by HPLC. CAS 77591-33-4. Lyophilized powder. For research use only. Bulk wholesale available with COA and global shipping.
Thymosin Beta-4 Peptide (Tβ4) – Full-Length 43-Amino Acid Sequence – ≥98% Purity Lyophilized Powder – For Research Use Only
Thymosin Beta-4 (Tβ4), also known by the recommended International Nonproprietary Name (INN) timbetasin, is a naturally occurring 43-amino acid peptide found in virtually all human and animal cells . It is one of the most abundant members of the thymosin beta family, with the highest concentrations in platelets, leukocytes, and wound fluid .
The peptide is synthesized with an N-terminal acetylation (Ac-SDKP…), which enhances stability and biological activity compared to non-acetylated forms . This full-length sequence (Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser-OH) represents the complete native protein .
Scientific Background
Thymosin Beta-4 functions through a unique dual-mechanism profile :
| Mechanism | Description |
|---|---|
| Intracellular | Primary G-actin sequestering protein; binds to and sequesters actin monomers (G-actin), thereby inhibiting actin polymerization and regulating cytoskeletal dynamics |
| Extracellular | “Moonlighting” signaling molecule released at sites of injury to orchestrate tissue repair, cell migration, angiogenesis, and anti-inflammatory cascades |
The therapeutic rationale for Tβ4 is founded on its pleiotropic functions, which include:
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Pro-migratory – Promotes cell migration and adhesion
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Pro-angiogenic – Enhances capillary formation and pericyte recruitment
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Anti-inflammatory – Downregulates key inflammatory molecules
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Anti-apoptotic – Protects cells from corrosive or oxidative damage
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Anti-fibrotic – Reduces excessive scar tissue formation
Key Features
| Feature | Detail |
|---|---|
| 🧬 Sequence (Full-Length) | Ac-SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES |
| 🔬 Sequence (Short Form) | Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser-OH |
| ⚖️ Molecular Formula | C₂₁₂H₃₅₀N₅₆O₇₈S |
| ⚖️ Molecular Weight | 4,963.44 Da |
| 🧪 Purity | ≥98% (HPLC) – ≥99.5% available from premium GMP sources |
| 🔢 CAS Number (Full-Length) | 77591-33-4 |
| 🔢 CAS Number (Alternative) | 77642-24-1 (also cited for Tβ4) |
| ❄️ Storage (Powder) | -20°C (stable for up to 3 years) |
| ❄️ Storage (Solution) | -20°C or -80°C; avoid repeated freeze/thaw cycles |
| Available Formats | Lyophilized powder in vials (1mg, 2mg, 5mg, 10mg, bulk) |
| Research Applications | Actin polymerization studies, cell migration assays, angiogenesis research, wound healing models, inflammation studies, ocular repair research |
Research Applications
| Application Area | Description |
|---|---|
| 🧫 Actin Sequestration Studies | Investigating G-actin binding and cytoskeletal dynamics; Tβ4 is the primary intracellular actin-sequestering peptide |
| 🔬 Angiogenesis & Vasculogenesis | Endothelial cell migration, capillary formation, and pericyte recruitment studies |
| 🩹 Wound Healing Models | In vitro and in vivo tissue repair research; Tβ4 promotes cell migration and reduces inflammation |
| 👁️ Ocular Research | Corneal wound healing after burn or injury models; protective effects on corneal epithelial cells |
| 🔧 Cardiovascular Research | Vascular development, arteriogenesis, and endothelial-mesenchymal transition studies |
| 🧪 Inflammation & Immunology | Downregulation of inflammatory molecules; immune system responsiveness studies |
Clinical Development Status
Thymosin Beta-4 has been investigated in multiple clinical trials for conditions including :
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STEMI (heart attack)
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Dry Eye Syndrome
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Diabetes
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Pressure Ulcers
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Corneal Wound Healing
Clinical trials have included Phase 2, Phase 3, and Phase 4 studies, with active and recruiting status as of 2024-2025 .
Specifications – Bulk/Wholesale Format
| Specification | Detail |
|---|---|
| Product Form | Lyophilized (freeze-dried) white powder |
| Purity | ≥98% (HPLC) – ≥99.5% available from GMP-certified sources |
| CAS Number | 77591-33-4 |
| Molecular Weight | 4,963.44 Da |
| Molecular Formula | C₂₁₂H₃₅₀N₅₆O₇₈S |
| Sequence Length | 43 amino acids (full-length native sequence) |
| N-Terminal Modification | Acetylated (Ac-) |
| Storage Temperature | -20°C (long-term); -80°C for extended storage |
| Shelf Life (Unopened) | 24-36 months at -20°C |
| Shelf Life (Reconstituted) | 7-14 days at 2-8°C; do not freeze/thaw repeatedly |
| Manufacturing Capability | Available from GMP-certified facilities (up to 200g per batch for 43-AA peptides) |
Handling & Reconstitution Instructions (For Research Use Only)
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Reconstitution: Add sterile water or bacteriostatic water to the lyophilized powder. A standard ratio is 1 mL water per 1 mg peptide.
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Mixing: Gently swirl or stir. Do not shake vigorously, as peptides are sensitive to mechanical stress.
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Storage After Reconstitution: Store at 2-8°C and use within 7-14 days. For longer storage, aliquot and store at -20°C or -80°C, avoiding repeated freeze-thaw cycles .
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For Difficult Dissolution: Add a small amount of DMSO first, then dilute with water.
Full Sequence Information
For researchers requiring sequence verification:
| Format | Sequence |
|---|---|
| One-Letter Code | Ac-SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES |
| Three-Letter Code | Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser-OH |
Key Structural Features:
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N-terminal acetylation (Ac-) – enhances stability
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43 amino acid residues
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Contains the actin-binding motif LKKTET (residues 17-23) – the same active domain found in TB-500
Comparison: Full-Length Tβ4 vs. TB-500 Fragment
| Feature | Full-Length Thymosin Beta-4 | TB-500 (Synthetic Fragment) |
|---|---|---|
| Sequence Length | 43 amino acids | 7 amino acids (Ac-LKKTETQ) |
| Molecular Weight | ~4,963 Da | ~889 Da |
| CAS Number | 77591-33-4 | 885340-08-9 |
| Biological Activity | Full native protein; all epitopes intact | Retains actin-binding domain only |
| Research Applications | Actin sequestration, angiogenesis, inflammation, wound healing | Actin-binding studies, simplified mechanism research |
Compliance & Disclaimer
⚠️ FOR RESEARCH USE ONLY. NOT FOR HUMAN OR VETERINARY USE.
This product is sold for laboratory research purposes only. It is not a pharmaceutical, drug, or medical device. Not intended for diagnostic, therapeutic, or in vivo human applications. Not for use in food, cosmetics, or household products.
Thymosin Beta-4 is a naturally occurring peptide and has been investigated in clinical trials, but this product is not for human administration. Any use in competitive animals or humans is strictly prohibited.
By purchasing this product, you affirm that you are a qualified research professional and that you will use this product only in lawful, ethical research applications.
Why Source From Us?
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✅ High-purity peptides (≥98% to 99.5% by HPLC)
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✅ GMP-certified manufacturing facilities (ISO9001, cGMP)
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✅ Large-scale synthesis capability (up to 200g per batch for 43-AA peptides)
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✅ Third-party COA (Certificate of Analysis) available for each batch
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✅ Bulk wholesale pricing – competitive rates for research quantities
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✅ OEM/ODM services available (custom purity, vial size, labeling)
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✅ Discreet worldwide shipping via DHL, FedEx, UPS
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✅ Free samples available for qualified research institutions
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✅ Refund/replacement policy for quality issues
Contact us for wholesale pricing, COA requests, bulk quotations, and shipping details. Long‑term research supply partnerships welcome.
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